COURSE | INTRODUCTION TO QUPATH SOFTWARE

09-06-2025

OVERVIEW | This course intends to give a brief introduction (Theoretical & Practical) to the use of QuPath (free open-source software for "Quantitative Pathology") for analysis of tile & stitched large image datasets, such as those obtained at slide scanners from the Histopathology and Bioimaging Units.

DATES & VENUE | 21st - 23rd May 2025 @ RISE-Health, University of Beira Interior, Covilhã (Portugal)

HYBRID | online plenary lectures (21st May) + practical sessions in person on each node + online tutorials (22nd-23rd May)

COST | Free

REGISTRATION DEADLINE | 11th May 2025, limited to 10 participants

REQUIREMENTS | Bring your own laptop + transformer + mouse / Course Material (Images + Models + Extensions) - on folder provided / Install QuPath [https://github.com/qupath/qupath/releases]


Participants will be notified of course acceptance on the 12th May 2025 by email.

If you have any questions, please contact us: rise-ubi.microscopia@fcsaude.ubi.pt.


PRELIMINARY PROGRAMME

Day 1 | 21st May

Session I - Introduction to image analysis

14h00 - 14h15 Presentation of PPBI

14h15 - 14h55 What is an Image?

14h55 - 15h35 Types of images, colormaps and stain vectors

15h35 - 15h50 Coffee Break

15h50 - 16h30 Concepts of thresholding, ML and DL

16h30 - 17h00 QuPath in the world of pathology


Day 2 | 22nd May

Session II - Introduction to QuPath

09h30 - 10h15 How to handle images on QuPath?

10h15 - 11h15 Tools - Annotations

11h15 - 11h30 Coffee Break

11h30 - 12h30 Tools - Detections

12h30 - 14h00 Lunch Break

Session III - Brightfield Images

14h00 - 14:45 Stain Vectors

14h45 - 15h45 Pixel Classification

15:45 - 16:00 Coffee Break

16:00 - 16:30 Object Classification

16h30 - 17:30 Density Maps


Day 3 | 23rd May

Session IV - Fluorescence Images

09h30 - 10:30 Multiplexed analysis

10h30 - 11h30 Object Classification

11h30 - 11h45 Coffee Break

11h45 - 12h15 Pixel Classification

Session V - Automated workflows

12h15 - 13:00 Introduction to groovy scripting

13h00 - 14h00 Lunch Break

Session VI - Call4Help (Optional)

14:00 - 16:00 Working on your images

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